|The screening visit (Visit 1) lasts 3 hours and involves 2 blood draws, a urine collection, a physical exam, a test to measure percentage of body fat, drinking a sugar drink and an electrocardiogram (EKG).
If eligible according to screening results, the next 2 visits are 36 hour-long inpatient stays. During this time, two IV lines are placed, blood is drawn, urine is collected, several fat biopsies are taken, vital signs are measured, 3 meals are provided and questionnaires are given. Additionally, on inpatient Day 1, we will give a very low dose of a drug (LPS) or a placebo, in order to learn more about the inflammatory effects that occur in people who are overweight. On inpatient Day 2, we will do an IV glucose tolerance test involving glucose and insulin administration, then the study is over and volunteers are discharged from the hospital. The second inpatient visit occurs approximately one month after the first and follows the same procedures and timeline as the first inpatient stay.
Prior to each inpatient stay, participants will have a telephone consultation with a dietitian.