Rodent Cardiovascular Phenotyping Core Request for Services

1.0 GENERAL INFORMATION

1.1 Requester and PI/Lab Manager information

1.2 Study questions

1.3 Billing source and details

2.0 REQUESTED SERVICES

Select Rodent Core Services to be provided. A price will dynamically update as you add services.

2.1 Echocardiographic Services:

Please enter the total number of echoes for all mice in study - NOT per-mouse.

Cost is per mouse/per time point. Non-invasive single transthoracic cardiac ultrasound exam performed under Avertin (tribromoethanol)-induced anesthesia.

Users can consider A for screening mice for homogenous decreases in function /hypertrophy (e.g. sepsis, etc.). Consider D in mice with regional wall motion abnormalities. Note that M Mode parameters do not accurately represent LV structure and function.



SCREENING ECHO
Acquisition and analysis of M-Mode images for LV function (fractional shortening), chamber dimensions, and wall thickness. Ideal for initial assessment of genetically-engineered mouse models.

SCREENING PLUS ECHO
Everything screening echo includes, plus acquisition and analysis of 2 dimensional images for LV end-diastolic and end-systolic volumes and ejection fraction.

ECHO for Advanced Diastolic Function Analysis*
Acquisition and analysis of 2D, M-Mode, and Doppler images to assess diastolic function including mitral valve inflow, tissue Doppler imaging, pulmonary vein flow, and cardiac strain (LV volumes, EF, LV Mass).

TAC ECHO
Screening Plus echo (M-Mode, volumes, EF) in addition to aortic trans-banding gradient assessment.

MI ECHO
Acquisition of 2D long axis images for cardiac strain (LV volumes, EF, LV Mass) and 2D short axis serial imaging slices for regional wall motion analysis.

TAC+ MI (Heart Failure) ECHO
MI echo plus aortic trans-banding gradient assessment.

Right heart assessment (PAB ECHO)
Acquisition of 2D and Doppler images for assessment of RV area, RV free wall thickness, RV tissue Doppler imaging, TAPSE, tricuspid regurgitation, and pulmonary artery band gradient.



*Consultation with Core required prior to scheduling.

2.2 Cardiac Surgical Services

TRANSVERSE AORTIC CONSTRICTION (TAC)- Compensated LV Hypertrophy Model
Operative placement of constriction on transverse aorta that results in left ventricular hypertrophy with normal systolic function in timeframe of 1-4 weeks. Timing of endpoint determined by scientific question. Recommend M-mode echo with gradient at endpoint.

MYOCARDIAL INFARCTION (MI)
Operative placement of a permanent occlusion around the left anterior descending artery of the mouse heart. Includes recording mouse weight. Size of MI can be customized but is typically sufficient size to impact LV function. Recommended Complete 2D echo and collection of endpoints at 1-2 weeks post-surgery.

OPEN CHEST ISCHEMIA REPERFUSION INJURY (OCIR)
Operative placement of an occluder around the left anterior descending artery for 30-45mins of occlusion followed by reperfusion for typically 24hrs. Vital dye staining will also be performed which involves euthanasia and placement of infusion line in aorta, and ligation of innominate artery, carotid artery and left subclavian artery. Heart will then be perfused with tetrazolium chloride and Evans Blue, with analysis to determine infarcted area and “area at risk”.

COMBINED TRANSVERSE AORTIC CONSTRICTION (TAC)/MYOCARDIAL INFARCTION (MI) – Heart Failure (HF) Model
Operative placement of constriction on the transverse aorta combined with a small myocardial infarction caused by permanent ligation of the distal third of the left anterior descending artery. Heart Failure (global LV dilatation and remodeling) will typically progress over a 4 week period. Recommend Complete 2D echo at endpoint.

PULMONARY ARTERY BANDING (PAB)
Operative placement of constriction on the pulmonary artery. RV dilation, dysfunction, and tricuspid regurgitation will typically progress over a 7-10 day period. We recommend a PAB echo be performed at day 10 post surgery.

PRE-WEANING AORTIC BANDING (PWAB)
Operative placement of constriction on the ascending aorta at 3 weeks of age. PWAB results in left ventricular hypertrophy. Recommended 8-10 week post surgery echo with systolic and diastolic assessment.

OTHER SERVICES

INJECTIONS OF PHARMACOLOGICAL AGENTS

JUGULAR CATHETERIZATION (for drug infusion)

ALZET PUMP IMPLANTATION

TELEMETRY IMPLANTATION

ORGAN HARVEST

2.3 Hemodynamic Services

HEMODYNAMICS I
Millar catheter placement, in carotid/aorta and recording of arterial or aortic pressures only.

HEMODYNAMICS II
Millar catheter placement in left or right ventricle, and derivation of ventricular peak pressures, ventricular end-diastolic pressure, +/- dP/dt, and tau.

HEMODYNAMICS III
Millar catheter placement into left ventricle and recording of LV measurements as in “Hemodynamics II” during pharmacological

HEMODYNAMICS IV
Millar catheter placement into left ventricle, Pressure-Volume loop recording including all measurements of “Hemodynamics II”.

3.0 TERMS AND SUBMISSION

Please review the terms below and submit your form to the Core. We will respond withing 48 hours of your submission.

Terms:

1) Prior IACUC approval must be obtained by investigator and provide copy of approved protocol to Core.

2) Investigator is responsible for transferring animals to the animal housing facility 1-141B on 1st floor of SCTR. Animals should be transferred at least one day prior to scheduled echo date. The CVI core will be responsible for pick up and return of the animals to 1-141B.

3) Once the transfer is initiated, please fill out the “CVI Mouse Phenotyping Core Mouse and Data Transfer Logsheet” excel found on website. This excel will be used throughout the duration of the study to communicate back and forth on transfer of mice, procedure dates, mortality and echo data transfer. Mortality must be reported to the Mouse Cardiovascular Phenotyping Core.

4) Housing costs/per diems are the full responsibility of the investigator.

5) The Mouse Cardiovascular Phenotyping Core will perform the 1st post-operative check, unless other arrangements have been made.

6) Charges may be subject to change as the cost of supplies increase.

7) If the animal dies or data cannot be acquired, the animal will be subtracted from the total cost.

8) “Penn Cardiovascular Institute’s Rodent Cardiovascular Phenotyping Core (RRID: SCR_022419)" should be cited in publications arising from the services. For echo services, please acknowledge our S10: S10OD016393.

By checking the box below, I agree to follow the terms and conditions described in this application. I understand the terms and fees for the services and I agree to pay the invoices from the Penn Cardiovascular Institute’s Mouse Cardiovascular Phenotyping Core for the services I have requested: